Exercise Induced Changes in Fat Metabolism

Summary

Participation Requirements

Description

General study design Subjects who exercise regularly will be instructed to exercise exactly 3 days before the experimental trial, and then abstain from their exercise training program until after the experiment is complete. Subjects will arrive to the Substrate Metabolism Laboratory (1210 CCRB) afte...

General study design Subjects who exercise regularly will be instructed to exercise exactly 3 days before the experimental trial, and then abstain from their exercise training program until after the experiment is complete. Subjects will arrive to the Substrate Metabolism Laboratory (1210 CCRB) after at least a 6h fast. After resting quietly in the laboratory for approximately 30min, Dr. Horowitz will collect a resting blood sample by venipuncture, as he has done in our other IRB approved protocols (e.g., HUM00029179). Briefly, Dr. Horowitz will wash his hands and put on gloves. The participant's skin will be disinfected at the venipuncture site with isopropyl alcohol. Single-use needles will be used for each participant and immediately discarded into a biohazard sharps container after use. After the blood draw, direct pressure will be applied to the site of needle insertion to avoid/reduce bruising and discomfort. Subjects will exercise (bike or treadmill) for 1h at a moderate exercise intensity (approximately 65% of their predicted VO2peak). This moderate exercise intensity has been used extensively by Dr. Horowitz's laboratory in previous exercise testing and training studies in both lean and obese individuals, and is well tolerated by both populations. During the exercise session, subjects will wear a heart rate monitor to ensure participants are exercising at the correct exercise intensity. Subjects will also use the mouthpiece and nose clip (described above) for ~5min at the beginning, middle, and end of exercise to verify they are exercising at approximately 65% of their predicted VO2peak. A study team member will be present with the participant at all times during exercise to ensure the comfort and safety. Immediately after exercise another blood sample will be collected.

Tracking Information