Therapeutic Effect of Sodium Selenite on Oxidative Stress in Patients With Severe Sepsis
Last updated on April 2022Recruitment
- Recruitment Status
- Completed
Inclusion Criteria
- sepsis, severe sepsis or septic shock, APACHE II score between 19 and 24 points after admission
- sepsis, severe sepsis or septic shock, APACHE II score between 19 and 24 points after admission
Exclusion Criteria
- mechanical ventilation for less than 24h, neuromuscular disease and terminal illness were also excluding criteria
- mechanical ventilation for less than 24h, neuromuscular disease and terminal illness were also excluding criteria
Summary
- Conditions
- Severe Sepsis
- Type
- Observational
- Design
- Observational Model: Other
- Time Perspective: Prospective
Participation Requirements
- Age
- Between 20 years and 80 years
- Gender
- Both males and females
Description
Patients will be divided into two groups. Se-group who received selenium supplementation during hospitalization in the form of sodium selenite pentahydrate at 750 mg/day for 6 days immediately after admission to our department (1000 mg of sodium selenite pentahydrate = 333 micrograms of selenium) (S...
Patients will be divided into two groups. Se-group who received selenium supplementation during hospitalization in the form of sodium selenite pentahydrate at 750 mg/day for 6 days immediately after admission to our department (1000 mg of sodium selenite pentahydrate = 333 micrograms of selenium) (Selenase, Vivax; selenium hereinafter). The placebo group will receive continuous saline NaCl 50 ml/day for 6 days as a continuous infusion (excluding additional infusion therapy). Patients will be further divided into subgroups according to OI on the day of admission to ICU. The oxygenation index (OI) PaO2/FiO2 (partial pressure of oxygen in arterial blood/ fraction of inspired oxygen) ratio in patients will be calculated daily. The determination of biochemical and hematological parameters of blood samples will be as a part of the routine diagnostic methods. Data collection for the Se-AOX study will be performed at two-day intervals: T1 (1st-2nd day), T2 (3rd-4th day) and T3 (5th-6th day). Kinetic methods for estimating the activities of glutathione peroxidase (GPx, E.C. 1.11.1.9), glutathione reductase (GR, E.C.1.6.4.2) will be performed using a kit (Sigma-Aldrich, Germany) and that of superoxide dismutase (SOD, E.C. 1.15.1.1) by means of the SOD-Assay Kit-WST (Fluka, Japan) following the user manual provided.
Inclusion Criteria
- sepsis, severe sepsis or septic shock, APACHE II score between 19 and 24 points after admission
- sepsis, severe sepsis or septic shock, APACHE II score between 19 and 24 points after admission
Exclusion Criteria
- mechanical ventilation for less than 24h, neuromuscular disease and terminal illness were also excluding criteria
- mechanical ventilation for less than 24h, neuromuscular disease and terminal illness were also excluding criteria
Tracking Information
- NCT #
- NCT02026856
- Collaborators
- Not Provided
- Investigators
- Not Provided
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