Recruitment

Recruitment Status
Completed
Estimated Enrollment
200

Inclusion Criterias

Regular antenatal examination from the first trimester;
Give birth in the university hospital (The 1st affiliated hospital of Sun Yat-sen University)
Women with singleton pregnancy;
Regular antenatal examination from the first trimester;
Give birth in the university hospital (The 1st affiliated hospital of Sun Yat-sen University)
Women with singleton pregnancy;

Exclusion Criterias

Taking any drug that affect glucose and lipid metabolism and insulin sensitivity.
Younger than 18 years old;
Diagnosed DM before pregnancy;
...
Taking any drug that affect glucose and lipid metabolism and insulin sensitivity.
Younger than 18 years old;
Diagnosed DM before pregnancy;
Complicated with other diseases such as hypertension, eclampsia, thyroid diseases, etc.;
Multiple pregnancy;
Older than 40 years old;

Summary

Conditions
Gestational Diabetes Mellitus
Type
Observational
Design
  • Observational Model: Case-Control
  • Time Perspective: Cross-Sectional

Participation Requirements

Age
Between 18 years and 40 years
Gender
Only females

Description

First,30 pregnant women with GDM and 60 pregnant control women with normal glucose tolerance (NGT) matched for maternal and gestational age were enrolled in the study. All the subjects underwent antepartum screening in the First Affiliated Hospital of Sun Yat-sen University. Blood samples were obtai...

First,30 pregnant women with GDM and 60 pregnant control women with normal glucose tolerance (NGT) matched for maternal and gestational age were enrolled in the study. All the subjects underwent antepartum screening in the First Affiliated Hospital of Sun Yat-sen University. Blood samples were obtained after overnight fasting at the time of oral glucose tolerance test (OGTT). Serum FGF19 and FGF21 levels were determined by enzyme-linked immunosorbent assay (ELISA) and were correlated with anthropometric, metabolic, and endocrine parameters. Homeostasis model assessment (HOMA-IR) index was calculated and analysed. Second, samples for measurement were obtained from 30 women with GDM and 35 healthy pregnant controls undergoing caesarean sections at term. mRNA and protein expression levels of FGF19/FGF21 and their co-receptor βKlotho(KLB)in placenta, rectus muscle and subcutaneous fat tissues were investigated with real-time quantitative polymerase chain reaction (qRT-PCR), western-blot and immunohistochemistry (IHC), respectively. Clinical data were collected and analysed. Data were analyzed by SPSS 20.0 database. The results were expressed as mean ± standard deviations or median with interquartile range. Differences between groups were assessed by Student's unpaired t test, Mann-Whitney U test, or Chi-square test as appropriate. Correlation analysis was performed using the Spearman rank correlation method. To identify independent relationships and adjust the effects of covariates, multiple linear regression analyses were performed. P values of <0.05 were considered significant.

Inclusion Criterias

Regular antenatal examination from the first trimester;
Give birth in the university hospital (The 1st affiliated hospital of Sun Yat-sen University)
Women with singleton pregnancy;
Regular antenatal examination from the first trimester;
Give birth in the university hospital (The 1st affiliated hospital of Sun Yat-sen University)
Women with singleton pregnancy;

Exclusion Criterias

Taking any drug that affect glucose and lipid metabolism and insulin sensitivity.
Younger than 18 years old;
Diagnosed DM before pregnancy;
...
Taking any drug that affect glucose and lipid metabolism and insulin sensitivity.
Younger than 18 years old;
Diagnosed DM before pregnancy;
Complicated with other diseases such as hypertension, eclampsia, thyroid diseases, etc.;
Multiple pregnancy;
Older than 40 years old;

Locations

Guangzhou, Guangdong, 510080
Guangzhou, Guangdong, 510080

Tracking Information

NCT #
NCT01858597
Collaborators
Not Provided
Investigators
Study Director: Zilian Wang, M.D., PhD Obstetrics and Gynechology Department of the 1st affiliated hospital of Sun Yat-sen University