Cell Distribution in Induced Sputum in Patients With Asthma

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Objectives: The aim of this study is the characterization of healthy children/ young adults and matched patients with asthma in terms of lung function, and the degree of the systemic and bronchial inflammation. Sputum and serum samples are analyzed by quantitative real-time polymerase chain reaction...

Objectives: The aim of this study is the characterization of healthy children/ young adults and matched patients with asthma in terms of lung function, and the degree of the systemic and bronchial inflammation. Sputum and serum samples are analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and by a cytometric bead assay (CBA). Components of the innate immune system (mannose-binding protein, TLR recognition proteins and surfactant proteins) are determined from sputum or blood respectively. Methods and Work Programme: This study consists of two study visits (V1 and V2) V1 (day 0 - duration 1 hour): Measurement of exhaled nitric oxide (eNO) Lung function testing with and body plethysmography Blood test: blood count, CRP, RAST, serum inflammatory mediators, markers of the non-specific pulmonary defense system Induced sputum attained by an ultrasonic-nebulizer for inflammatory mediators and microbiological investigations V2 (day 14-28 - duration 1 hour): Measurement of nitric oxide in expired air (eNO) Lung function testing with spirometry and body plethysmography Induced sputum attained by a nozzle-nebulizer for inflammatory mediators and microbiological investigations Study population: Children, adolescents and young people (6 - 25 years of age). Both the patients and the healthy subjects are recruited from the outpatient clinic of Pediatric Allergy and Pulmonology.

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